Millipore Corp.

Aug. 27, 2007
Sample Prep Devices for Ultra HPLC Offer High Resolution

New ultra-high pressure liquid chromatography (UHPLC) methods provide higher resolution than traditional HPLC methods. In order to take full advantage of this superior resolution, it has become increasingly important that samples, mobile phases and buffers are properly prepared.  

Millipore provides syringe filters, membranes and holders in many configuration that are designed and manufactured to maximize UHPLC system performance. These devices minimize column clogging and have low extractables, low binding, and low hold-up volumes to optimize sample preparation. Lab water systems are also available that provide ultrapure water for all aspects of UHPLC from mobile phase preparation to standards, blanks and samples. 

A sampling of Millipore’s UHPLC-compatible products include:

Millex Syringe Filters

These filters ensure minimal signal-to-noise ratios and clean baselines.  They have a broad chemical compatibility and low hold-up volumes making them a convenient means of clarifying and/or removing small particles from samples prior to UHPLC analysis.

Millipore Express Membrane

Millipore Express Plus membrane, the first asymmetric PES membrane available for ultra-fast filtration in laboratory applications, consists of polyethersulfone (PES) and enables the rapid filtration of additives, buffers and other aqueous solutions. Millipore Express Plus membrane is available as 25 mm and 47 mm cut disks with 0.22 µm pore sizes as well as Steritop vacuum filter cups and syringe filter devices.

Milli-Q water with an Advantage A10 monitor and Q-POD dispenser

This ultrapure water system is designed to conveniently deliver high quality ultrapure water.  The compact production unit may be placed on or under the bench, while the Q-POD unit polishes and delivers water at the point of use.  Up to three Q-POD units may be attached to any one production unit at different locations throughout the laboratory.

MultiScreen Solvinert Plates

These plates are optimized for drug discovery applications, including total drug analysis.  They come in both deep well and standard volumes and are available with a choice of either chemically resistant hydrophobic or hydrophilic PTFE membranes.  They demonstrate low binding, low extractables and high recoveries.