Thermo Electron Corp.

Sept. 14, 2004
Fast, Efficient HPLC Separations above 200 degrees C
This manufacturer has developed a new porous graphitic carbon medium for reversed phase liquid chromatography that allows the technique to be used safely at temperatures up to 200°C, beyond the reach of modified silicas, which can be damaged when used above 60 to 80°C.Running separations at higher temperatures reduces solvent viscosity, enhancing mass transfer between mobile and stationary phases, the manufacturer claims. High-temperature processing also improves efficiency, providing sharper peaks and increased peak capacity, and provides more sensitivity than traditional HPLC due to improved peak height and better signal-to-noise ratio. In addition, separations are faster because, at high temperature, back pressure is reduced, so flow rates can be higher.