Pall Life Sciences

March 4, 2009
Advanced Technology For Protein Purification Reduces Processing Costs, Saves Time

Pall Life Sciences, a global leader in filtration, separation and purification, has introduced the most advanced chromatography sorbents for processing large amounts of protein during the capture and intermediate steps of purification. The Pall Q and S HyperCel ion exchange sorbents also contribute significantly to cost and time savings for laboratory to production scale applications. Pall Life Sciences is a business of Pall Corporation.

The new Pall sorbents with Q and S chemistries share the same HyperCel media platform with the company’s mixed-mode sorbents (MEP, HEA and PPA HyperCel).  HyperCel sorbents are structured on a mechanically- and chemically-stable base matrix designed for protein capture and impurity removal. The Q and S sorbents, which are suitable for laboratory and process-scale use, help process recombinant proteins, monoclonal antibodies, plasma derivatives and other proteins from various feedstock.
“The continuous increase of protein expression titers and bioreactor feedstock volumes created new challenges that called for the improvement of downstream purification processes,” said David Lescinski, vice president, chromatography, Pall Life Sciences. “We discovered that the new Q and S ion exchange chromatography sorbents, backed by Pall’s extensive validation documentation and technical support, will deliver the desired protein purification throughput while helping reduce process time along with reducing costs for buffers, effluent disposal, sorbents and equipment.”

Pall Q and S HyperCel sorbents will debut during IBC Life Sciences’ Biopharmaceutical Development & Production Week (La Costa Resort & Spa, Carlsbad, California, March 2-6) and also will be displayed at INTERPHEX 2009 (Jacob K. Javits Convention Center, New York City, March 17-19).

Pall’s new Q and S HyperCel sorbents:

  • Can be operated at high flow rates with industry-standard columns and systems. The sorbents deliver high throughput attributed to flow performance and high dynamic binding capacities (>150 mg/mL of protein at two minutes residence time). Faster binding and elution reduces processing time and enhances the plant throughput.
  • Bring new selectivities for ion exchange separations and new options to separate target protein from contaminants. Binding and elution in moderate salt conditions also may contribute to a better separation between target protein and impurities.
  • Can minimize salt concentration to desorb bound protein, meaning less subsequent processing is required for the next downstream processing step -- buffer exchange, dilution, or diafiltration.
  • Deliver a proven sorbent for large scale applications, with easy packing and handling compatible with industry-standard columns and systems. The sorbents are operated at high flow rates (500-600 cm/hr) and low backpressure (typically < 1.5 bar in 40 cm ID columns).
  • Contribute to lowering downstream processing costs and enhanced process economics.

The new Pall sorbents are available in a variety of packaging configurations, including convenient 1 ml pre-packed Pall PRC columns designed for fast methods optimization and selectivity screening.