A “Global” Method for the Determination of Tissue Thiols, Disulfides and Thioethers Using an HPLC System Incorporating a Novel Boron-Doped Diamond (BDD) Detector

Presented here is a preliminary method illustrating the ability to separate and detect a number of thiols, disulfides and thioethers in under 30mins. Peak authenticity was evaluated by spiking samples with external standards and by treatment of the plasma sample with the reducing agent tris[2-carboxyethyl phosphine] [TCEP] to convert disulfides into thiols.

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